|
种属 |
人 |
|
别称 |
SNF96.2; sNF96-2 |
|
组织来源 |
施万细胞 |
|
疾病 |
1型神经纤维瘤病Nf1 |
|
传代比例/细胞消化 |
1:2传代, |
|
完全培养基配置 |
DMEM培养基 ；10%胎牛血清 ；1%双抗 |
|
简介 |
sNF96.2 is a Schwann cell line that was isolated in 1996 from a 28-year-old, male patient with neurofibromatosis type 1 Nf1.The sNF96.2 cell line was derived from a recurrent mass associated with nerve and diagnosed as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were derived from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. |
|
形态 |
雪旺细胞样 |
|
生长特征 |
贴壁生长 |
|
STR |
Amelogenin X CSF1PO 12 D5S818 11 D7S820 10,11 D13S317 10 D16S539 11 TH01 6 TPOX 11 vWA 17,19 |
|
倍增时间 |
~33 h |
|
培养条件 |
气相：空气，95%；二氧化碳，5%。 温度：37摄氏度，培养箱湿度为70%-80%。 |
|
冻存条件 |
冻存液：90%FBS，DMSO 10%, 或使用非程序冻存液：官网货号JY-H040 |
|
保藏机构 |
ATCC; CRL-2884 |
|
产品使用 |
仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。 |
